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The cells were then washed three times with PBS containing 1% BSA (w/v) followed by 1 h incubation with 1:1,000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark
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The direct glucagon-mediated stimulation of hepatic beta-oxidation produces liver fat effects that exceed what would be predicted from weight loss alone
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Similar results could be observed in female LTRKO mice treated with T3 (Supplementary Fig
