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Consistently, these mutational effects of the backbone peptide P28, P29 and S34 residues were conserved with amylin receptor 2/3 receptor ECD binding (Figure 3 and Table 2)

LDH assay Non-specific membrane disruption was assessed quantitatively by measuring the lactate dehydrogenase (LDH) activity in the medium using the cytoscan-LDH cytotoxicity assay kit (G-Biosciences Inc., St Louis, Mo, USA)

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The instrument was tuned for optimal detection of lipid molecules ( m / z 2002000) in both polarities using the quadrature phase detection (QPD) (2 ) mode