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This includes weightlifting, jumping or jogging, and running

To generate the sgRNA plasmids, primers were designed with the CRISPR DESIGN tool ( and inserted into the pDD162 vector (Addgene #47549) using the site-directed mutagenesis kit (TOYOBO SMK-101)

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[PMC free article: PMC10852395] [PubMed: 37523624] Special Considerations for the Treatment of Children With Soft Tissue Sarcoma Cancer in children and adolescents is rare, although the overall incidence has slowly increased since 1975.[1] Children and adolescents with cancer should be referred to medical centers that have a multidisciplinary team of cancer specialists with experience treating the cancers that occur during childhood and adolescence