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Description
The mixture was dispensed into 6-well plate and incubated at 37 C and 5% CO 2 allowing the collagen to polymerise
The crude residue was purified by silica gel flash column chromatography ( n -heptane:EtOAc, 2:1) yielding 4-nitrophenyl (2-(pyridin-2-yldisulfaneyl)ethyl) carbonate (89%)
The practical routine: finish your evening tasks, prepare for bed, inject DSIP, then complete your remaining pre-sleep routine (brushing teeth, dimming lights, reading)
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