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While chronically high insulin levels can be problematic (e.g., in insulin resistance or type 2 diabetes), a short-term, post-meal spike is normal and necessary

Following a single oral dose of 0.5g, the maximum plasma concentration in healthy subjects is 48.5 mol/L

UHPLC-MS/MS analyses The plasma lipid extracts were analyzed by dual-channel UHPLC (Vanquish Duo, Thermo Scientific) coupled to a TSQ Altis triple-stage quadrupole mass spectrometer (Thermo Scientific) with electrospray ionization source operating in both negative and positive modes