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Firstly, we detected the expression and location of FOXO4 in mice testes by immunohistochemical staining and found that FOXO4 was rarely expressed in young mice testes, but expressed typically in the Leydig cells of aged mice (Figure 6A)

When activated, multiple growth factors, including TGF-1, IGF-1, platelet-derived growth factor, vascular endothelial growth factor and epidermal growth factor are released from -granules of platelets, with regenerative effects (Marx, 2001)

These concerns necessitate further research, including dedicated long-term safety studies in animal models, specifically designed to assess potential risks associated with higher doses and prolonged exposure

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