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Membranes were washed three times for 5 min with PBST (0.2% Tween 20) and incubated with secondary antibody (dilution, 1:3000) for 1 hour

Nanoparticles have gained significant attention as carriers for peptide delivery due to their unique properties, including high surface area, tunable size, and surface modifications, which will lead to a reduction in the drug side effects and an increase in the drugs efficacy