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Reconstitution refers to adding a measured volume of sterile or bacteriostatic water to the vial in order to create a solution with a known concentration, expressed as international units (IU) per millilitre (mL)

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Additionally, it can be caused by either a lack of B12 in the diet, gastritis, pernicious anemia, digestive diseases (e.g., Crohn's disease and celiac disease), surgery on the GI tract, alcohol use disorder, transcobalamin II deficiency, or Sjgren's syndrome [9]

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Data from the Dutch hunger winter of 194445 suggested a nutritional basis