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Lysis buffer containing protease and phosphatase inhibitors was added to the samples, and the protein concentration was measured using a BCA assay (Beyotime, China)

Silva et al

This binding could modulate astrocytic glycogen storage and lactate production, thereby translating peripheral metabolic information into changes in local neural circuit activity and influencing feeding behavior

Alpha lipoic acid is included for its role in the bodys energy processes

N-Acetyl-L-cysteine ethyl ester exhibits enhanced cell permeability, and produce NAC and cysteine
