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Total cellular RNA was extracted from 2.5 10 5 cells with the RNeasy Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturers instructions

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After GGT removes the glutamyl group from GSH, cysteinylglycine is cleaved by an extracellular dipeptidase to the constituent amino acids cysteine and glycine, and the released amino acids are transported into the cell via dedicated transporters

preclinical data support anti-CD19 CAR-Ts with 4-1BB, and BCMA-expressing B cell targeting (APRIL-based CAR-T) prolongs survival in SLE models, underpinning ongoing clinical exploration (49, 50)