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Cancer Cell Int2022 Oct 7;22(1):304

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Here, we describe the development of AlphaScreen assays to identify small molecule inhibitors of enzymes and protein-protein interactions using the highly miniaturized 1536-well format

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A high SAM/SAH ratio leads to efficient and effective methylation, resulting in SAM-mediated allosteric inhibition of the enzyme, which reduces the concentration of 5-methyl-THF and decreases the activity of the methionine cycle, and thus also the production of SAM [21]