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2.13 In vivo tumorigenesis study To establish non-small cell lung cancer xenografts in nude mice, a total of 5 10 6 A549 cells expressing the indicated shRNA or constructs were stably transfected with either control, or the indicated shRNAs targeting RanBPM were harvested, washed with PBS, suspended in 100 L of PBS, and injected into the right flank of each mouse (n = 6 per group)