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RT-qPCR was carried out using the 2 Hieff UNICON Universal Blue qPCR Master Mix (Yeasen, Shanghai, China) under the following amplification conditions: Uracil-DNA Glycosylase (UDG) activation at 50 C for 2 min, initial denaturation at 95 C for 2 min, then 40 cycles of 95 C for 15 s and 60 C for 30 s for denaturation and annealing/extension, respectively, concluding with a melting curve analysis

Heat also reduces your bodys sebum production levels, helping to keep your follicles and pores clear

2013;15:R39

doi: 10.1359/jbmr.1998.13.3.325

Mobile phase A comprised a mixture of 40 mM sodium acetate and 17% methanol, while mobile phase B was 100% methanol