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Moreover, the unintentional disruption of natural antisense transcripts (NATs) present in the non-coding strand of DNA during knockout generation further complicates the matter as they participate in various cellular regulatory processes via the cis or trans mechanisms, for instance, Cftr gene knockout mouse ( Cftr / ) which was generated by inserting an in-frame mutation in exon 10, to produce a truncated protein [47]

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[DOI] [PMC free article] [PubMed] [Google Scholar] 86.Cui J.-J., Huang Z.-Y., Xie Y.-H., Wu J.-B., Xu G.-H., Li C.-F., Zhang M.-M., Yi L.-T

Injection-Based Administration Subcutaneous injections: All GLP-1 RAs are administered via subcutaneous injection Prefilled pens: Most common delivery system, allows for self-administration Auto-injectors: Simplify the process for patients with dexterity issues Multi-dose pens: Require dose dialing and needle attachment Dosing frequency: Daily: Liraglutide (Saxenda, Victoza), some formulations of exenatide (Byetta) Weekly: Semaglutide (Wegovy, Ozempic), tirzepatide (Mounjaro, Zepbound), dulaglutide (Trulicity), exenatide extended-release (Bydureon) Titration schedules: Most FDA-approved GLP-1 RAs require gradual dose escalation to minimize gastrointestinal side effects