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doi: 10.1016/S0378-5173(03)00039-5

Furthermore, decreased bacterial richness has been linked to high carbohydrate 73 and high-fat/low-protein concentrations 74 , as found in the lean and Western diets, respectively, the latter result only in old-aged rats 74

The amount depends on your desired concentration

[3] The same work profiled shifts in the cecal microbiome associated with treatment
Microneedling the scalp before AHK-Cu application can substantially improve penetration
