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Considering the position of the Gly 1228, we could argue that the mutation G1228D will trigger a steric hindrance with T998 that is tightly constrained by F583 and cannot move easily in the apo and LTC 4 -bound conformations

The final fluorescence signal was normalized by cell number
EVIDENCE GRADES Multiple rat studies (Krivic 2006 PMID 16583442

Incubation was carried out overnight (12 h) at 4 C under gentle rotation
