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In addition, nuclear pores do not restrict diffusion of low molecular weight solutes like GSH [14], which could possibly difficult the establishment of a specific pool of GSH within the nucleus

All of them contain glutathione as a key ingredient

Furthermore, the biological activity and neuroinductive properties of GDNF are highly dependent on its production system and post-translational modifications, with mammalian cell-produced GDNF showing superior efficacy compared to bacterial systems (Shamadykova et al., 2021)

The plate were then incubated at 37 C, with 5% of CO 2 and 95% of humidity during 45 min, after which 50 L of reaction mixture was added to each well and incubated for additional 30 min at room temperature in the dark