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Additionally, various signaling pathways and related proteins play important roles in hair follicle regeneration, among which the Wnt signaling pathway is the classical example

Aside from declining with age, reduced glutathione can also be caused by stress, poor nutrition, and exposure to certain toxins

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In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min