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Investigated in body-composition biomarker research under supervised protocols

Understanding the potential side effects of MOTS-c is equally important before considering its use

Cell culture and treatment U251 cells (from ATCC, Manassas, VA, USA) were cultured at 37 C in 5% CO 2 in high glucose DMEM and 10% fetal bovine serum supplemented with penicillin (100 U/ml) and streptomycin (100 g/ml)

This reduces material waste, supports procedural efficiency, and lowers the risk of accidental microbial introduction during repeated laboratory manipulation

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