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Transfer the supernatant to a new tube, and add ddH 2 O to reduce the SSA concentration to 0.5% for the assay

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Mohanty RR, Padhy BM, Das S, Meher BR (2021) Therapeutic potential of N-acetyl cysteine (NAC) in preventing cytokine storm in COVID-19 review of current evidence

This work was supported by the National Natural Science Foundation of China (82472525 and 82272567), Jiangsu Medical Research Project (ZD2022014), Program of Suzhou Health Commission (GSWS2022002), National and Local Engineering Laboratory of New Functional Polymer Materials (SDGC2205), Foundation of National Center for Translational Medicine (Shanghai) SHU Branch (SUITM202403), the Project of MOE Key Laboratory of Geriatric Diseases and Immunology (KJS2502), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX25_1798), Suzhou Municipal Medical and Health Science and Technology Innovation Project (SKY2021023), and Ke Jiao Xing Wei Plan of Wujiang District (WWK202005)

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