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HMGB1 can also be considered as an alarmin in myocardial tissues which exacerbates the pathological characteristics involving DCM by inducing the release of cytokines, promoting chemotaxis of immune cells, activating immune cells, endothelial cells and fibroblasts and serving as an adjuvant in the production of autoantibodies

Frhlich, R

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Then, to verify the impact on ENS neuronal activity, intestinal preparations were subjected to electrical field stimulation (EFS) at increasing frequencies with constant voltage, to induce neuronal depolarization and consequent neurotransmitters release