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Drugs developed based on this hypothesis, however, have not met with much success in recent times

Together, these fuel inflammatory cascades and metabolic dysfunction

Iron is stored within ferritin

Mechanistically, luteolin induces G0/G1 cell-cycle arrest through downregulation of critical cell-cycle regulators, including CCNE1, CDK2, and CDK4

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]