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The number of contacts between residues 8-28, embedded inside of the membrane, and POPE lipids on average decreased by around 20 contacts for the control group: at the beginning of the trajectory, there were 121 contacts which fell to an average of 104(14) contacts in the last 50 ns of the trajectory

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There is, however, emerging evidence that uORF-containing transcripts are in addition coordinately regulated by non-canonical re-initiation factors

The stable CHO-K1 cell line expressing Flag-tagged MC4R chimera-Cb80 was cultured in HamF12/DMEM (1:1) supplemented with 5% FBS, 8 mM Glu, and under 0.5 mg/ml G418 geneticin selection