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The concentration of HNE in human serum is 0.05-0.15 M under physiological conditions (15)

Antioxidants normally terminate many reactions by removing free radical intermediates and inhibit other oxidation reactions

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Then, fluorescence (Ex: 340/380 nm, Em: 515 nm) was recorded in an IonOptix spectrofluorometer (IonOptix LLC, Westwood, MA)

Regarding in vitro studies, the exposure of human (SK-N-SH) and mouse (Neuro-2a) neuroblastoma cells to silica NPs (SiNPs) (10 g/mL/24 h) raised the intracellular content of A in both cell lines, which was associated with increased APP and reduced NEP protein levels