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Blood (~5 mL) was collected in BD K 3 EDTA-containing vacutainer tubes (Fisher Scientific, Pittsburgh, PA) through an indwelling venous catheter before and at 0.25, 0.5, 1, 1.5, 2, 3, 4, 6, 8, and 12 hours following drug administration

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The tripeptide backbone (Gly-His-Lys) coordinates a single copper ion through its histidine and amine groups, which is why GHK-Cu is grouped with the copper peptides rather than the unliganded GHK sequence