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Distinct TAM subsets display variable responses to iron metabolism, with iTAMs exhibiting high iron content and an enrichment of heme and iron metabolism-related genes

For some experiments, the suspension of multilamellar vesicles underwent a one-step extrusion process, and was therefore immediately frozen and thawed five times before being extruded through a polycarbonate filter of 0.2 m pore size

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To increase the protein recovery, the pellet from this step was resuspended in a buffer (containing 7 M urea and 2 M thiourea in 0.4 M triethylammonium bicarbonate at pH 8.5), freeze-thawed, vortexed and centrifuged at 15,000 g for 15 min at 20 C

doi: 10.3390/cimb45120588