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GLT-1 cKO exhibits depressive phenotypes accompanied by an elevation of ROS/RNS in the mPFC, while the activity of GS and the levels of Glu-Gln appear to remain unchanged

Before LCMS analysis, the dried extracts were reconstituted in 100 L of ACN:H 2 O (1:1, v/v), sonicated for 10 min, and centrifuged for 15 min at 16,200 g and 4 C to remove insoluble debris

They feed directly into the Krebs cycle, sustaining oxidative phosphorylation even when glucose metabolism falters

Zhang Q, Ramracheya R, Lahmann C, Tarasov A, Bengtsson M, Braha O, Braun M, Brereton M, Collins S, Galvanovskis J, Gonzalez A, Groschner LN, Rorsman NJ, Salehi A, Travers ME, Walker JN, Gloyn AL, Gribble F, Johnson PR, Reimann F, Ashcroft FM, Rorsman P

In: Therapeutic Insights into Herbal Medicine Through the Use of Phytomolecules , p 32