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The quantification of gene expression by qPCR was calculated using the Ct method

J Anim Sci Biotechnol

Doxycycline hydrochloride has been applied in studies related to breast cancer, prostate cancer, bladder cancer, and other cancers [3]

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Most studies report an increased sensitivity to experimental pain and, therefore, a decreased pain threshold in depressed compared to non-depressed subjects especially when emotional aspects of experimental pain are taken into account (Ushinsky et al., 2013)