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HY-K0301, MCE, USA) was added to each well and incubated for 2 h before the absorbance at 450 nm was read on a microplate reader (SpectraMax M2, Molecular Devices, USA)

5.2 Flavoprotein functional defect-related mutation mechanisms In addition to genes directly involved in riboflavin uptake and metabolism, numerous inherited disorders arise from structural or functional defects in flavoproteins themselves

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With increasing doses of Zfp423, as expected, increasing binding of Zfp423 was observed

Abrencillo R, Mira-Avendano IC, Estrada-Y-Martin RM, Palacio D, Kuyumcu G, Debiane LG, Peralta AR, Cohen A, Simoff MJ, Mehta V, Diaz-Mendoza J, de Groot PM, Truong MT , Moran CA