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Briefly, diluted serum with sample silution buffer by the ratio of 1: 1

The biotinylated proteins are then purified using streptavidin-agarose resin and selectively eluted with 2-mercaptoethanol (2-ME) to break the disulphide linkage in biotin-HPDP

The Role of N-Acetylcysteine (NAC) If you have ever looked into glutathione support, you have likely seen NAC mentioned

We sincerely thank all collaborating authors for their valuable contributions, insightful discussions, and collective efforts throughout the preparation of this manuscript